intragastric administration  (MedChemExpress)

Journal: BMC cancer

Article Title: Cholesterol-induced colorectal cancer progression and its mitigation through gut microbiota remodeling and simvastatin treatment.

doi: 10.1186/s12885-025-14379-3

Figure Lengend Snippet: Fig. 3 Lactobacillus plantarum-mediated reduction of serum cholesterol alleviates CRC tumor growth. (A) In vivo bioluminescence imaging showing tumor progression in four groups: I (Lactobacillus plantarum + Normal diet), II (Normal diet), III (Antibiotics), and IV (Antibiotics + Lactobacillus plantarum). Antibiotics group: 0.02mL/g ceftriaxone intragastric administration every two days. (B) Quantification of fluorescence intensity representing tumor size across the four groups. (C) Serum cholesterol and LDL/VLDL expression levels (µg/µl), showing a significant reduction with Lactobacillus plantarum supplementation. (D) Histological analysis of colon tissues from each group, with HE staining showing tissue architecture and Ki-67 staining highlighting proliferating cells. (E-G) Relative expression levels of inflammatory markers IL-2, TNF-α, and IFN-γ, respectively, in colon tissues across the four groups. Data are presented as mean ± SEM, with significant differences indicated (*,P < 0.05; **, P < 0.01; ***, P < 0.001)

Article Snippet: In the antibiotic depletion group, the model mice were given 0.02 mL/g ceftriaxone intragastric administration every two days, and the concentration of ceftriaxone sodium was 0.6 g/mL (MCE, HY-B0712).

Techniques: In Vivo, Imaging, Fluorescence, Expressing, Staining

Journal: Scientific Reports

Article Title: Intervention effect of regulating GABA-A receptor activity on the formation of experimental abdominal aortic aneurysm in rats

doi: 10.1038/s41598-024-82913-7

Figure Lengend Snippet: Diameter of abdominal aorta of rats in each Group ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\bar{x}$$\end{document} ± s).

Article Snippet: Randomly divided into sham operation group (saline perfusion + saline infiltration, n = 10), Model group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration, n = 10), topiramate group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + topiramate 100 mg/kg/d intragastric administration, Janssen Pharmaceutical Xian, H20020555, n = 10), bicuculline group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + bicuculline 2 mg/kg/d intraperitoneal injection, MCE, HY-N0219, n = 10).

Techniques:

Journal: Scientific Reports

Article Title: Intervention effect of regulating GABA-A receptor activity on the formation of experimental abdominal aortic aneurysm in rats

doi: 10.1038/s41598-024-82913-7

Figure Lengend Snippet: Dilatation degree of abdominal aorta and appearance of aneurysm in each group. ( A ) No dilation was observed in the perfused segment of the abdominal aorta in the Sham group. ( B ) Localized saccular aneurysm formation was observed in the perfused segment of the abdominal aorta in the Model group. ( C ) Localized mild dilation was observed in the perfused segment of the abdominal aorta in the Topiramate group. ( D ) Irregular aneurysm formation was observed in the perfused segment of the abdominal aorta in the Bicuculline group.

Article Snippet: Randomly divided into sham operation group (saline perfusion + saline infiltration, n = 10), Model group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration, n = 10), topiramate group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + topiramate 100 mg/kg/d intragastric administration, Janssen Pharmaceutical Xian, H20020555, n = 10), bicuculline group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + bicuculline 2 mg/kg/d intraperitoneal injection, MCE, HY-N0219, n = 10).

Techniques:

Journal: Scientific Reports

Article Title: Intervention effect of regulating GABA-A receptor activity on the formation of experimental abdominal aortic aneurysm in rats

doi: 10.1038/s41598-024-82913-7

Figure Lengend Snippet: Comparison of elastic fiber content, collagen fiber content, and smooth muscle cell content in the abdominal aorta of rats in each group ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\bar{x}$$\end{document} ± s).

Article Snippet: Randomly divided into sham operation group (saline perfusion + saline infiltration, n = 10), Model group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration, n = 10), topiramate group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + topiramate 100 mg/kg/d intragastric administration, Janssen Pharmaceutical Xian, H20020555, n = 10), bicuculline group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + bicuculline 2 mg/kg/d intraperitoneal injection, MCE, HY-N0219, n = 10).

Techniques: Comparison

Journal: Scientific Reports

Article Title: Intervention effect of regulating GABA-A receptor activity on the formation of experimental abdominal aortic aneurysm in rats

doi: 10.1038/s41598-024-82913-7

Figure Lengend Snippet: Histopathological changes of abdominal aorta of rats in each group. ( A ) The vascular wall structure in the Sham group remains intact. ( B ) In the Model group, the vessel wall is thinned, with discontinuous, partially lost or fragmented elastic and collagen fibers, and a reduction in smooth muscle cells. ( C ) In the Topiramate group, the vessel wall is partially thinned, but clear inner and outer elastic layers are still visible, and smooth muscle cells are arranged in an orderly fashion. ( D ) In the Bicuculline group, the vessel wall is significantly thinned, with degradation of the extracellular matrix, smooth muscle cell loss, and dissection formation. ( E ) Quantitative analysis of normalized vascular wall thickness. ( F ) Differences in vascular wall thickness across individual rats.

Article Snippet: Randomly divided into sham operation group (saline perfusion + saline infiltration, n = 10), Model group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration, n = 10), topiramate group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + topiramate 100 mg/kg/d intragastric administration, Janssen Pharmaceutical Xian, H20020555, n = 10), bicuculline group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + bicuculline 2 mg/kg/d intraperitoneal injection, MCE, HY-N0219, n = 10).

Techniques: Dissection

Journal: Scientific Reports

Article Title: Intervention effect of regulating GABA-A receptor activity on the formation of experimental abdominal aortic aneurysm in rats

doi: 10.1038/s41598-024-82913-7

Figure Lengend Snippet: Comparison of IL-4, IFN-γ, and IL-6 concentrations in rat plasma and abdominal aorta among different groups ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\bar{x}$$\end{document} ± s)

Article Snippet: Randomly divided into sham operation group (saline perfusion + saline infiltration, n = 10), Model group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration, n = 10), topiramate group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + topiramate 100 mg/kg/d intragastric administration, Janssen Pharmaceutical Xian, H20020555, n = 10), bicuculline group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + bicuculline 2 mg/kg/d intraperitoneal injection, MCE, HY-N0219, n = 10).

Techniques: Comparison, Clinical Proteomics

Journal: Scientific Reports

Article Title: Intervention effect of regulating GABA-A receptor activity on the formation of experimental abdominal aortic aneurysm in rats

doi: 10.1038/s41598-024-82913-7

Figure Lengend Snippet: Expression of GABA α5 subunit in inflammatory cells in the abdominal aortic wall across different groups. ( A ) Widespread expression of the GABA α5 subunit in infiltrating inflammatory cells in the Model, Topiramate, and Bicuculline groups. ( B ) Quantitative analysis of GABA α5 subunit-positive inflammatory cells, * P < 0.05 compared to the Model group, n = 10. ACS, aortic cross section. ( C ) Representative immunofluorescence images showing colocalization of CD3 and GABA α5 subunit.

Article Snippet: Randomly divided into sham operation group (saline perfusion + saline infiltration, n = 10), Model group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration, n = 10), topiramate group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + topiramate 100 mg/kg/d intragastric administration, Janssen Pharmaceutical Xian, H20020555, n = 10), bicuculline group (Elastase 5U perfusion + 1.0 mmol/L calcium chloride infiltration + bicuculline 2 mg/kg/d intraperitoneal injection, MCE, HY-N0219, n = 10).

Techniques: Expressing, Immunofluorescence